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INPUT   DATA

♥ EXAMPLE Of Input/Output

Title

Initial cell concentration, Ni		cells/mL
Growth constant, K		m-1
Final cell concentration, N		cells/mL
Final time, T		min

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OUTPUT   VARIABLES   &   GRAPHS

Variables	Values	Units
♦  Final cell concentration, N		cells/mL	Graphs:    Cell concentration Vs. Time
♦  Final time, T		min
♦  Generation time, Tg		min

Bacterial Growth Curve

Cell growth of bacteria or other unicellular organisms refers to cell division and increase in cell quantity. When cells from an overnight culture are inoculated into a fresh medium, they experience four growth phases: lag, exponential, stationary and death. The exponential phase is usually modeled by the logarithmical expression:

     log₁₀N = log₁₀N_i + K.T

The Generation Time, the time required for cells in a culture to double in number is derived as:

     T_g = [log₁₀2]/K

where
     N_i = initial cell concentration
     N = desired cell concentration
     T = time to attain desired cell concentration
     T_g = generation time
     K = constant exponential growth factor, which depends on the medium, available nutrients, temperature, aeration level and genotype

Tips

    ◊ You should enter either the final concentration to determine the final inoculation time, or enter the final time to determine the final concentration. You cannot enter both final concentration and time.

    ◊ Use link EXAMPLE Of Input/Output  to demo data entry expectations and results; you may edit & use it as starting point

BIBLIOGRAPHY

• Stephenson FH; Calculations For Molecular Biology And Biotechnology: A guide to mathematics in the laboratory; Academic Press, An Imprint of Elsevier, Amsterdam, 2003