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INPUT   DATA

♥ EXAMPLE Of Input/Output

Title

Number of "A" nucleotides		integer
Number of "C" nucleotides		integer
Number of "G" nucleotides		integer
Number of "T" nucleotides		integer

Phosphorylated   Dephosphorylated

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OUTPUT   VARIABLES   &   GRAPHS

Variables	Values	Units
♦  Total Molecular Weight		daltons

MW Of Single-Stranded DNA Sequence

A DNA sequence is a succession of letters representing the primary source of a real or hypothetical DNA molecule or strand. The possible letters are A, C, G, T, represnting the four nucleotide subunits of a DNA strand - Adenine, Cystosine, Guanine and Thymine bases covalently linked to phospho-backbone.

For small-stranded DNA molecules, such as synthetic oligonucleotides, the molecular weight of the constituent nucleotides can be added to determine the strand's total molecular according to the following formula:

     MW = 335.2n_A + 311.2n_C + 351.2n_G + 326.2n_T + P

where
     MW = total molecular weight
     n_i = number of nucleotides of subunit i (i = A, C, G or T)
     P = constant [ = -101.0 for dephosphorylated (lacking an end phosphate group), = +40.0 for phosphorylated]

Tips

    ◊ Use link EXAMPLE Of Input/Output  to demo data entry expectations and results; you may edit & use it as starting point

BIBLIOGRAPHY

• Stephenson FH; Calculations For Molecular Biology And Biotechnology: A guide to mathematics in the laboratory; Academic Press, An Imprint of Elsevier, Amsterdam, 2003