MELTING TEMPERATURE OF DNA STRAND
INPUT DATA
Title
Length of amplicon, L
bp
"G+C" fraction in amplicon, F
G+C
%
Concentration of potassium ions, [K
+
]
M
Concentration of magnesium ions, [Mg
2+
]
M
RESULTS
Variables
Values
Units
♦ Salt concentration, [S]
0.25
M
♦ Melting temperature, T
m
92.268
°C